Data for flow cytometry, FlowCam, and microscopy comparison experiment

Published: 2018-06-11

This data set was collected from two minicosm experiments conducted at Davis Station, Antarctica. 1. Variance experiment - 2013/14 summer season 2. Ocean acidification experiment - 2014/15 summer season It includes: - description of methods for all data collection and analyses. - environmental data logged throughout the experiment; nutrients, temperature, light climate. - flow cytometry counts; autotrophic cells, heterotrophic nanoflagellates, and prokaryotes. - FlowCam counts; individual phytoplankton species data. - microscopy counts; individual phytoplankton species data. Context from the public summary for project 4026: Manmade CO2 has increased ocean acidity by 30% and it is projected to rise 300% by 2100. Antarctic waters will be amongst the earliest and most severely affected by this increase. Microbes are the base of the marine food chain and primary drivers of the biological pump. This project will incubate natural communities of Antarctic marine microbes in minicosms at a range of CO2 concentrations to quantify changes in their structure and function, the physiological responses that drive these changes, and provide input to models that predict effects on biogeochemical cycles and Antarctic food webs.